Shanghai Yaxin Biotechnology Co.,Ltd.
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Place of Origin: | Zhejiang, China (Mainland) |
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Sequencing Grade Chymotrypsin / Chymotrypsin / SRCT / sequencing grade enzyme
CAS: 9004-07-3
EC: 3.4.21.1
SOURCE:Expressed in E. Coli, purified by HPLC.
DESCRIPTION
Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene
sequence is same as that human chymotrypsin. The recombinant chymotrypsin hydrolyzes at the carboxyl
side of aromatic amino acids: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at
Leu and Met. Chymotrypsin activity is optimal in the pH range of 7.0–9.0.
MAIN FEATURES
Source |
E. Coli |
Purity |
NLT 95% by HPLC analysis. |
Physical form |
Lyophilized powder |
Specific activity |
NLT 1500 USP units/mg pro |
Contaminant activity |
No carboxypeptidase A, or other proteases contaminant. |
APPLICATION
Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or
MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.
This sequencing grade enzyme can be used alone or in combination with other proteases to produce.
RECOMMEND USAGE
To prepare 1-10mg/ml with 1ml 1mM HCl, used within 2 days, or stored below -20 after repacked
STORAGE AND STABILITY
Recommend recombinant Chymotrypsin lyophilized powder stored under 2-8 in sealed container. It is
stable within 24 months. After dissolved, it should be stored under -20.
RELATED PRODUCT
Recombinant Trypsin (sequencing purity);
Recombinant Carboxypeptidase B (sequencing purity);
Recombinant Chymotrypsin (sequencing purity);
V8 (sequencing purity).