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Sequencing Grade Chymotrypsin for digestion reactions in-solution 

Place of Origin: Zhejiang, China (Mainland) 
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Product Detail

Model No.: SRCT03

 
Sequencing Grade Chymotrypsin / Chymotry

 

Sequencing Grade Chymotrypsin / Chymotrypsin / SRCT / sequencing grade enzyme

 

CAS: 9004-07-3


EC: 3.4.21.1

 

SOURCE:Expressed in E. Coli, purified by HPLC.

 

DESCRIPTION

 

Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene

 sequence is same as that human chymotrypsin. The recombinant chymotrypsin hydrolyzes at the carboxyl

 side of aromatic amino acids: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at

 Leu and Met. Chymotrypsin activity is optimal in the pH range of 7.0–9.0. 

 

MAIN FEATURES

 

Source

E. Coli

Purity

NLT 95% by HPLC analysis. 

Physical form

Lyophilized powder

Specific activity

NLT 1500 USP units/mg pro

Contaminant activity

No carboxypeptidase A, or other proteases contaminant. 

 

APPLICATION

 

Protein digests for peptide mapping applications or protein identification by peptide mass fingerprinting or

 MS/MS spectral matching. It is suitable for digestion reactions in-solution or in-gel.

This sequencing grade enzyme can be used alone or in combination with other proteases to produce.

 

RECOMMEND USAGE

 

To prepare 1-10mg/ml with 1ml 1mM HCl, used within 2 days, or stored below -20 after repacked 

 

STORAGE AND STABILITY

 

Recommend recombinant Chymotrypsin lyophilized powder stored under 2-8 in sealed container. It is

 stable within 24 months. After dissolved, it should be stored under -20. 

 

RELATED PRODUCT

 

Recombinant Trypsin (sequencing purity);

Recombinant Carboxypeptidase B (sequencing purity); 

Recombinant Chymotrypsin (sequencing purity);

V8 (sequencing purity).

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Company Info

Shanghai Yaxin Biotechnology Co.,Ltd. [China (Mainland)]


Business Type:Manufacturer
City: Shanghai
Province/State: Shanghai
Country/Region: China (Mainland)

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