Recombinant enzyme human chymotrypsin proteolytic enzyme CAS 9004-07-3 EC 3.4.21.1 RCT10 manufacturer from China Shanghai Yaxin Biotechnology Co.,Ltd.

Recombinant enzyme human chymotrypsin proteolytic enzyme CAS 9004-07-3 EC 3.4.21.1

Place of Origin:

Zhejiang, China (Mainland)


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Product Detail

Model No.: RCT10

Recombinant Chymotrypsin</p

Recombinant Chymotrypsin

DESCRIPTION

Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, purified with HPLC, the gene

sequence is same as that human chymotrypsin. The recombinant chymotrypsin hydrolyzes at the carboxyl

side of aromatic amino acids: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at

Leu and Met. Chymotrypsin activity is optimal in the pH range of 7.0–9.0. This sequencing grade enzyme

can be used alone or in combination with other proteases to produce protein digests for peptide mapping

applications or protein identification by peptide mass fingerprinting or MS/MS spectral matching. It is

suitable for digestion reactions in-solution or in-gel.

ADVANTAGES

Animal origin free:The use of recombinant chymotrypsin eliminates the content of other proteins, the

risk of virus presence, and other potential adventitious agents found in animal-derived chymotrypsin.

Stable:A sterile recombinant chymotrypsin lyophilized powder eliminates the contamination risks and

decreases the chance of activity loss in the process of transport and storage.

High purity:1)Recombinant Chymotrypsin provides increased specificity and eliminates contaminating

activities found in enzymes purified from pancreas.

2) No anion trypsin or cation trypsin.

3) One single main band at 25kD by SDS-PAGE.

SPECIFICATION

Serine endopeptidase that specifically hydrolyzes peptide bonds at the C-termini of Tyr, Phe, and Trp. Leu,

Met, Ala, Asp, and Glu are cleaved at a lower rate. Acts also upon amides and esters of susceptible amino

acids.

APPLICATION

Hydrolysis of proteins by chymotrypsin alone or in combination with other proteases. Suitable for peptide

mapping, fingerprinting, and sequence analysis.

For peptide digestion, use a ratio (w/w) of approximately 1:60 for chymotrypsin:peptide. Perform peptide

digests in 100 mM Tris HCl containing 10 mM CaCl2, pH 7.8, at 30 °C. Self digestion may occur if

temperatures above 37 °C are used. A known peptide such as melittin should be used as a control for all

experiments.

MAIN FEATURES

Source	E. Coli
Purified by	HPLC
Physical form	White lyophilized powder
Specific activity	≥1000 unit/mg pro
Purity	> 99%

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Company Info

Shanghai Yaxin Biotechnology Co.,Ltd. [China (Mainland)]

Business Type:Manufacturer
City: Shanghai
Province/State: Shanghai
Country/Region: China (Mainland)

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