Shanghai Yaxin Biotechnology Co.,Ltd.
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Place of Origin: | Zhejiang, China (Mainland) |
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alkaline stable protein A,colloidal gold and radioactive markers
Recombinant Protein A / Protein A / RSPA / rSPA / Lyophilized powder
SOURCE:E.Coli, HPLC purified.
SEQUENCE:Protein A mutant of Staphylococcus aureus
MOL. WEIGHT:25 kDa
FORM:Lyophilized powder;Solution:50mg/ml in deionized water or PBS.
PH STABLE RANGE:pH 1-13
APPLICATION:
Recombinant Protein A
The recombinant Protein A is a genetically engineering protein containing IgG-binding domains.
Recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds
to most human and mouse IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2, IgG2a, IgG2b,
IgG3). It also binds to cow, guinea pig, hamster, house, pig and rabbit total IgG form.
Recombinant protein A can be coupled to solid separation medium (such as agarose) for monoclonal and
polyclonal antibody purification. Recombinant protein A can be coupled to a variety of molecules (such as
fluorescent molecules, enzyme markers, biotin, colloidal gold and radioactive markers). These coupled
derivatives can be used in antibody test in the process of Western-blot, ELISA or immunohistochemical
tests.
PRODUCT SPECIFICATION
1. Test/Method Specification
2. Bioburden No organisms detected.
3. 12% SDS-PAGE Single major band
4. Coomassie Stain 25kD
5. UV Spectrum E270/250≥1.2, E0.1% at 270nm=0.14-0.20
6. UV spectrometry No DNA or RNA detected
7. Triton Content No Triton contaminant.
USAGE:
For Research or Manufacturing Purpose Only. Not for Humans.
Figure 1. A.Schematic structure of antibodies
B. Structure of SPA binding to IgG Fc Fragment
C. Immobilized SPA binding to IgG
Figure 2. SDS-PAGE of YaxinBio Recombinant Protein A.
PRODUCT INFORMATION
Product |
Cat.No |
Protein Content |
Packaging |
Manufacturer |
Recombinant Protein A |
RSPA25 |
≥97% |
10mg,100mg,1g or bulk |
YaxinBio |
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Web:http://www.yaxinbio.com Email:Zhaozhi@yaxinbio.com