Shanghai Yaxin Biotechnology Co.,Ltd.
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Place of Origin: | Zhejiang, China (Mainland) |
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recombinant human trypsin lyophilized powder,human trypsin supplier
Recombinant Human Trypsin
DESCRIPTION
Human Trypsin
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine
and arginine amino acid residues. Recombinant Human Trypsin is a genetically engineered protein,
expressed in E.Coli and purified by high pressure liquid chromatography. The amino acid sequence is the
same as Human Trypsin 2 sequence. The pH optimum of trypsin is 7 - 9. The enzyme is inhibited by serine
protease inhibitors, e.g. PMSF, and by metal chelating agents, e.g., EDTA. There are no contaminating
enzyme activities such as from carboxypeptidase A and chymotrypsin. No protease inhibitors such as
PMSF are contained in the preparation.
ADVANTAGES
Animal origin free:The use of recombinant Human Trypsin eliminates the risk of virus presence, and of any
other potential adventitious agents found in animal-derived trypsin. YaxinBio Recombinant Human Trypsin belongs to the AOF level 3.
Recombinant human trypsin:The amino acid sequence is the same as the Human Trypsin 2 sequence.
Stable:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases
the chance of activity loss in the process of transport and storage.
High purity:
contaminating activities found in lower purity enzymes.
2) No other contaminating proteases such as chymotrypsin or carboxypeptidase A.
3) One single main band at 24kD by SDS-PAGE.
Figure 1. SDS Page of YaxinBio Recombinant Human Trypsin (rhT)
Source | E. Coli |
Purified by | HPLC |
Physical form | White or White-like lyophilized powder |
Specific activity | ≥2500 USP u/mg pro. |
Purity | > 99% |
Contaminant activity | No chymotrypsin, carboxypeptidase A, and other protease contaminant. |
MAIN FEATURES
PRODUCT INFORMATION
Product | Cat. No. | Specific activity | Packaging | Manufacturer |
Recombinant Human Trypsin 2 | RHT03 | ≥2500 USP u/mg pro | 10mg,100mg or bulk | YaxinBio |
UNIT DEFINITION:One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a
reaction volume of 3.0ml at pH7.6 and 25, with BAEE as a substrate (1cm light path).
RELATED PRODUCTS
Recombinant Porcine Trypsin - Recombinant Trypsin Cell Disassociated Solution